In the present study. we attempted to control the pH profile of the catalytic activity of the industrially relevant alkaline protease KP-43. by incorporating 3-nitro-l-tyrosine and 3-chloro-l-tyrosine at and near the catalytic site. Thirty KP-43 variants containing these non-natural amino acids at the specific positions were synthesized in Escherichia coli host cells with expanded gen... https://missouriquiltcoes.shop/product-category/finishing-touches/
Variants of the industrially relevant protease KP-43 with suppressed activity under alkaline conditions developed using expanded genetic codes
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